By Shin J. Oh
As a certified operating within the frontlines of tissue prognosis and in daily perform, you would like a reference that provides you useful details in an easy-to-use layout. Containing over three hundred images, micrographs, and line drawings, together with over 60 colour illustrations, colour Atlas of Nerve Biopsy Pathology offers a transparent photograph of favourite ideas for nerve biopsies. You get new diagnostic options and clinicopathological correlations all in a framework designed for fast reference and straightforward studying. supplying all of the useful info you wish from A to Z, colour Atlas of Nerve Biopsy Pathology describes how to:perform a nerve biopsyhandle specimensprocess the nerve through frozen sections, through paraffin sections, and by way of semi-thin sectionsThe booklet delineates the overall pathological beneficial properties of peripheral nerve issues and the diagnostic potential and obstacles of the nerve biopsy. It info the common pathological good points of every affliction by means of providing colour photos and instance instances that supply a quick medical historical past and pertinent electrophysiological and different laboratory information. The exhaustively illustrated colour layout provide you with fast info in achieving a definitive prognosis. not like different books in this topic, this one includes frozen part fabrics, case histories, teased nerve illustrations, and newly defined neuropathies. the writer brings twenty-five years of expertise within the lab and educating to the advance of this atlas. within the lab or within the lecture room, at the entrance traces of tissue analysis, colour Atlas of Nerve Biopsy Pathology will give you the knowledge you wish in actual fact, concisely, and with ease.
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Extra info for Color Atlas of Nerve Biopsy Pathology
The nerve specimen is cut into two pieces, one-third for the transverse section and two-thirds for the longitudinal section. 1 Advantages and Disadvantages of Tissue Sections Section Type Frozen section Advantage Rapid diagnosis Population of myelinated fibers (modified trichrome) Detection of myelin digestion chambers (modified trichrome) Cresyl-fast-violet stain for metachromatic material Oil red O stain for lipid Relative ease of preserving the longitudinal sections straight for segmental demyelination Immunofluorescent studies Paraffin section Details of cells and anatomical structure Semithin section Population of myelinated fiber Detection of thinly myelinated fibers Detection of clustering of regenerated fibers Detection of onion-bulb formation Axon–myelin relationship EM section The only test for the unmyelinated fibers Widely spaced myelin (WSM) Uncompacted myelin lamellae (UML) Schwann cell inclusions Macrophage-induced demyelination or axonal change Teasing fiber Best method for differential diagnosis for axonal neuropathy vs.
Heat gently to dry. With the aid of low heat on a hot plate, flood sections with Paragon multiple stain solution** and sprinkle sodium borate lightly over staining. Staining is complete when a green sheen covers the staining surface. Wash well in warm water and flush slides with absolute alcohol. Store slides in an upright position in a slide spacer. Do not mount to avoid any wrinkling. Results appear as follows: Collagen — magenta to pink Cytoplasm — blue Nuclei — dark blue Myelin — very dark blue to black TOLUIDINE BLUE STAIN Everything is the same as above except a 1% toluidine blue solution† is used.
Thus, the course of the radial nerve is normally mapped with the nerve ©2002 CRC Press LLC Chapter 2 Final Proof 07/13/2001 7:58 AM Page 23 conduction study prior to a biopsy in order to guarantee the success of the nerve biopsy. The same principle of identification of the nerve is applied as described above. After the biopsy, the patient loses sensation over the territory of the superficial radial sensory nerve, including the first web space. REFERENCES 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Color Atlas of Nerve Biopsy Pathology by Shin J. Oh