By Prof.Dr. Wolfgang Rettig, Dr. Bernd Strehmel, Dr. Sigurd Schrader, Dipl.-Phys. Holger Seifert (auth.)
This interdisciplinary booklet offers a accomplished survey of the cutting-edge and on developments in fluorescence strategies in technological know-how, medication and engineering. the most chapters comprise prolonged studies that are supplemented via extra really good papers describing info and purposes. the quantity is meant for practitioners utilising this system of their regimen paintings and for researchers in and academia, either teams being lively in components comparable to environmental tracking, neuro and telephone biology, molecular medication, together with gene study, microscopy and immuno-assays, physics, fabric technology or chemsitry. Breathtaking new tools like ultra-fast time-resolved or unmarried molecule spectroscopy and correlation strategies, fluorescence probes, or imaging concepts and their purposes within the fields of electroluminescent polymers, visualization of membrane potentials in neurons or fluorescence imaging of the mind are defined via the world over popular authors.
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Extra resources for Applied Fluorescence in Chemistry, Biology and Medicine
IEEE 80: 869 - 889 44. Troy TL (1997) Biomedical Optical Imaging with Frequency Domain Photon Migration Measurements: Experiments and Numerical Image Reconstructions. PhD. Thesis, Purdue University, West Lafayette, IN, 1997 45. Burch CL (1993) Monte Carlo simulations of photon migration in highly scattering media. Masters Thesis, Vanderbilt University, Nashville, TN CHAPTER 2 Single-Molecule Detection in Biology with Multiplex Dyes and Pulsed Semiconductor Lasers M. Sauer, J. Wolfrum 1 Single-Molecule Detection with Far-Field Fluorescence Microscopy Techniques In recent times, researchers have made considerable efforts to achieve the detection of individual atoms and molecules in solids [1-6],on surfaces [7-10], and in liquids [11-20] using laser-induced fluorescence techniques.
Hence, from a statistical point of view, one is confronted with a problem of classification. An optimal way of classification has already been developed in the framework of information theory and is described in detail elsewhere [30-34]. For the time-resolved identification of single dye molecules in solution an important question arises: How many photons are necessary to distinguish two or more dye labels with different lifetimes? A first approximation for the probability of misclassification can be obtained by Cramers inequality .
Boas DA, O'leary MA, Chance B, Yodh AG (1994) Scattering of diffuse photon density waves by spherical inhomogeneities with turbid media: analytic solution and applications. Proc Natl Acad Sci USA 91: 4887 -91 34. O'leary M, Boas D, Chance B, Yodh A (1995) Experimental images of heterogeneous turbid media by frequency-domain diffusion-photon tomography. Optics Letters 20:426-428 35. Yao Y, Wang Y, Pei Y, Zhu W, Barbour RL (1997) Frequency-domain optical imaging of absorption and scattering distributions by a Born iterative method.
Applied Fluorescence in Chemistry, Biology and Medicine by Prof.Dr. Wolfgang Rettig, Dr. Bernd Strehmel, Dr. Sigurd Schrader, Dipl.-Phys. Holger Seifert (auth.)