Applied biochemistry and bioengineering - download pdf or read online

By Ichiro Chibata, Lemuel B. Wingard

ISBN-10: 0120411040

ISBN-13: 9780120411047

Immobilized Microbial Cells, quantity four presents an outline of the tools of immobilization, functions, and methods of using immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts.

This quantity is produced from seven chapters. It starts off with the ancient historical past of immobilized telephone study. next chapters specialise in the tools of immobilization and functions of immobilized microbial cells, residing cells, and organelles. The final chapters talk about fuel creation of immobilized cells for power new release and the chemical engineering research of immobilized-cell systems.

The ebook may be of significant use to chemists and chemical engineers.

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IV. THE CHEMISTRY OF ENTRAPMENT IN POLYMERIC CARRIERS Physical entrapment in porous polymeric carriers is undoubtedly the most widely used immobilization technique for whole cells. The size of immobilized species make it rather simple to prepare porous networks that combine complete cell retention with high porosity for substrate and product transport. The chemistry of such a network for­ mation, which inherently takes place in the presence of the cells to be immobilized (see Section II,B,3), can be classified along the following three different lines: 1.

As is well established in the field of heterogeneous catalysis, external and internal mass transfer can play an important role 46 Joachim Klein and Fritz Wagner in the overall reaction behavior. It is therefore highly advisable to consider transport phenomena in each practical catalyst development. A size-dependent or cell capacity-dependent reaction rate is a clear but indirect indication that transport steps are becoming rate control­ ling. In this context it is desirable to obtain direct information on the yield of immobilized activity (Eng, 1980; Klein et aL, 1981) and on the transport coefficients—that is, the effective diffusivity of the ratecontrolling substances (Klein and Washausen, 1979b; Klein and Man­ ecke, 1982).

The following performance profile can be given: 1. The covalent polymeric network is very stable under exceedingly variable reaction conditions. 2. The matrix is highly elastic and especially the PU foam is very compressible. The matrix seems especially suited for stirred sys­ tems; however, column packing is possible as well if the flow rate is low enough. 3. Blocks, flat sheets (membranes), granular particles, and beads can be obtained. In this sense PU is a very versatile system. 4. The foam has open macropores, whereas the matrix itself is rather dense.

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Applied biochemistry and bioengineering by Ichiro Chibata, Lemuel B. Wingard

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